ABSTRACT
<p><b>OBJECTIVE</b>To crosslink bovine pericardial valves by phthalocyanine sensitized photooxidation reaction.</p><p><b>METHODS</b>Fresh bovine pericardial tissues were immersed in PBS solution containing 0.01% cupreous sulfonatophthalocyanine and exposed to a broad wavelength light source for 20-120 h. Using sodium dodecyl sulfate-polyarylamide gel electrophoresis (SDS-PAGE) to analyze the cross-linking extent of pericardial tissue. With the help of attenuated total reflection-fourier transform infrared (ATR-FTIR), explained the change of protein structure after photooxidation reaction. Differential scanning calorimetry (DSC) was used to measure the change of shrinkage temperature. Tissue water content experiment method was also used.</p><p><b>RESULTS</b>Photooxidized tissue was resistant to extraction when it was compared with untreated tissue and had similar crosslinking extent over glutaraldehyde-treated tissue. The three dimension structure of collagen were changed but not seriously destroyed and the model of photooxidation reaction was validated. Phthalocyanine-treated tissue was much more flexible than glutaraldehyde-treated tissue.</p><p><b>CONCLUSION</b>Bovine pericardial tissue can be successfully crosslinked by phthalocyanine-mediated photooxidation reaction.</p>
Subject(s)
Animals , Cattle , Bioprosthesis , Cross-Linking Reagents , Electrophoresis, Polyacrylamide Gel , Heart Valve Prosthesis , Indoles , Pericardium , Chemistry , Spectroscopy, Fourier Transform InfraredABSTRACT
Glutaraldehyde clinically is the most commonly accepted crosslinking reagent for bioprosthetic valves preparation. Glutaraldehyde-treated tissue is stable against chemical and enzymatic degradation; however, its calcification and cytotoxicity are severe. Dye-mediated photooxidation is an alternative tissue preservation method that oxidizes the protein with visible light in the presence of a suitable photosensitizer. This article reviews chemical mechanism, research progress, clinical applications future development of these two methods.